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anti ulbp 1  (R&D Systems)


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    Structured Review

    R&D Systems anti ulbp 1
    Anti Ulbp 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ulbp+1/Human+ULBP-1+APC-conjugated+Antibody/pmc13046905-66-21-23
    Average 95 stars, based on 129 article reviews
    anti ulbp 1 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
    Article Snippet: Metformin was from Santa Cruz Technologies. .. Fluorescence coupled antibodies against human ICAM-1 (AF1730), ULBP-1 (MAB1380), MICA/B (MAB13001) and the blocking antibody against NKG2D (MAB139) were from R&D Systems; CD33, HLA and CD138 were from Beckman; DR4 (12-6644-73), DR5 (12-9908-42) and Fas (BMS140FI) were from eBiosciences and 7AAD from BD Biosciences. ..

    Blocking Assay:

    Article Title: Metformin sensitizes leukemic cells to cytotoxic lymphocytes by increasing expression of intercellular adhesion molecule-1 (ICAM-1)
    Article Snippet: Metformin was from Santa Cruz Technologies. .. Fluorescence coupled antibodies against human ICAM-1 (AF1730), ULBP-1 (MAB1380), MICA/B (MAB13001) and the blocking antibody against NKG2D (MAB139) were from R&D Systems; CD33, HLA and CD138 were from Beckman; DR4 (12-6644-73), DR5 (12-9908-42) and Fas (BMS140FI) were from eBiosciences and 7AAD from BD Biosciences. ..

    Activation Assay:

    Article Title: Targeting triple-negative breast cancer using cord-blood CD34⁺ HSPC-derived mesothelin-specific CAR-NKT cells with potent antitumor activity
    Article Snippet: Fluorochrome-conjugated antibodies specific for human iNKT TCR Vɑ24-Jβ18 (Clone 6B11, PE-conjugated, 1:20, cat. no. 552825) were purchased from BD Biosciences. .. Fluorochrome-conjugated antibodies specific for human fibroblast activation protein FAP (Clone 427819, PE-conjugated, 1:100, cat. no. FAB3715P), ULBP-1 (Clone 170818, PE-conjugated or unconjugated, 1:25, cat. no. FAB1380P or MAB1380), ULBP-2,5,6 (Clone 165903, APC-conjugated, 1:25, cat. no. FAB1298A), MSLN (Clone 420411, APC-conjugated, 1:20, cat. no. FAB32652A), and Nectin-4 (Clone 337516, PE-conjugated, 1:200, cat. no. FAB2659P), were purchased from R&D Systems. .. A goat anti-mouse IgG F(ab’)2 secondary antibody (cat. no. A-11001) was purchased from ThermoFisher.

    Article Title: Spatiotemporal profiling reveals distinct dynamics and checkpoint regulations of CAR-T and CAR-NKT cells against solid tumors
    Article Snippet: .. Fluorochrome-conjugated antibodies specific for human mesothelin (Clone 420411, PE-conjugated, 1:10, cat. no. FAB32652P, RRID: AB_ 1151946), fibroblast activation protein alpha (FAP; Clone 427819, PE-conjugated, 1:100, cat. no. FAB3715P-100, RRID: AB_3086725), ULBP-1 (Clone 170818, PE-conjugated, 1:25, cat. no. FAB1380P, RRID: AB_2687471), and ULBP-2,5,6 (Clone 165903, APC-conjugated, 1:25, cat. no. FAB1298A, RRID: AB_2257142) were purchased from R&D Systems. .. Fluorochrome-conjugated antibodies specific for mouse CD45 (Clone QA17A26, PE or APC-conjugated, 1:200, cat. no. 157604 or 157606, RRID: AB_2876536 or AB_2876537), F4/80 (Clone BM8, PE or FITC-conjugated, 1:100, cat. no. 123110 or 123108, RRID: AB_893486 or AB_893502), CD11b (Clone M1/70, APC or FITC-conjugated, 1:200, cat. no. 101212 or 101206, RRID: AB_312795 or AB_312789), Gr-1 (Clone RB6-8C5, PE or APC-conjugated, 1:200, cat. no. 108408 or 108412, RRID: AB_313373 or AB_313377), IgG2b, κ Isotype control (Clone MG2b-57, FITC or PE-conjugated, 1:50, cat. no. 401206 or 401208, RRID: AB_2783641 or AB_326637) were purchased from BioLegend.

    Article Title: Multimodal targeting of metastatic renal cell carcinoma via CD70-directed allogeneic CAR-NKT cells
    Article Snippet: Fluorochrome-conjugated antibodies specific for human iNKT TCR Vɑ24-Jβ18 (Clone 6B11, PE-conjugated, 1:20, cat. no. 552825) was purchased from BD Biosciences. .. Fluorochrome-conjugated antibodies specific for human fibroblast activation protein FAP (Clone 427819, PE-conjugated, 1:100, cat. no. FAB3715P), ULBP-1 (Clone 170818, PE-conjugated or unconjugated, 1:25, cat. no. FAB1380P or MAB1380), and ULBP-2,5,6 (Clone 165903, APC-conjugated, 1:25, cat. no. FAB1298A) were purchased from R&D Systems. .. Fluorochrome-conjugated antibodies specific for human TOX (Clone TXRX10, PE-conjugated, 1:50, cat. no. 12-6502-82) was purchased from eBioscience.

    Article Title: Targeting triple-negative breast cancer using cord-blood CD34⁺ HSPC-derived mesothelin-specific CAR-NKT cells with potent antitumor activity.
    Article Snippet: Fluorochrome-conjugated antibodies specific for human iNKT TCR Vɑ24-Jβ18 (Clone 6B11, PE-conjugated, 1:20, cat. no. 552825) were purchased from BD Biosciences. .. Fluorochrome-conjugated antibodies specific for human fibroblast activation protein FAP (Clone 427819, PE-conjugated, 1:100, cat. no. FAB3715P), ULBP-1 (Clone 170818, PE-conjugated or unconjugated, 1:25, cat. no. FAB1380P or MAB1380), ULBP-2,5,6 (Clone 165903, APC-conjugated, 1:25, cat. no. FAB1298A), MSLN (Clone 420411, APC-conjugated, 1:20, cat. no. FAB32652A), and Nectin-4 (Clone 337516, PE-conjugated, 1:200, cat. no. FAB2659P), were purchased from R&D Systems. .. A goat anti-mouse IgG F(ab’)2 secondary antibody (cat. no. A-11001) was purchased from ThermoFisher.

    Article Title: Spatiotemporal profiling reveals distinct dynamics and checkpoint regulations of CAR-T and CAR-NKT cells against solid tumors.
    Article Snippet: .. Fluorochrome-conjugated antibodies specific for human mesothelin (Clone 420411, PE-conjugated, 1:10, cat. no. FAB32652P, RRID: AB_ 1151946), fibroblast activation protein alpha (FAP; Clone 427819, PE-conjugated, 1:100, cat. no. FAB3715P-100, RRID: AB_3086725), ULBP-1 (Clone 170818, PEconjugated, 1:25, cat. no. FAB1380P, RRID: AB_2687471), and ULBP-2,5,6 (Clone 165903, APC-conjugated, 1:25, cat. no. FAB1298A, RRID: AB_2257142) were purchased from R&D Systems. .. Fluorochrome-conjugated antibodies specific for mouse CD45 (Clone QA17A26, PE or APC-conjugated, 1:200, cat. no. 157604 or 157606, RRID: AB_2876536 or AB_2876537), F4/80 (Clone BM8, PE or FITC-conjugated, 1:100, cat. no. 123110 or 123108, RRID: AB_893486 or AB_893502), CD11b (Clone M1/70, APC or FITCconjugated, 1:200, cat. no. 101212 or 101206, RRID: AB_312795 or AB_312789), Gr-1 (Clone RB6-8C5, PE or APC-conjugated, 1:200, cat. no. 108408 or 108412, RRID: AB_313373 or AB_313377), IgG2b, κ Isotype control (Clone MG2b-57, FITC or PE-conjugated, 1:50, cat. no. 401206 or 401208, RRID: AB_2783641 or AB_326637) were purchased from BioLegend.

    other:

    Article Title: Multimodal targeting of metastatic renal cell carcinoma via CD70-directed allogeneic CAR-NKT cells.
    Article Snippet: Fluorochrome-conjugated antibodies specific for human TOX (Clone TXRX10, PE-conjugated, 1:50, cat. no. 12-6502-82) was purchased from eBioscience.

    Transfection:

    Article Title: A genetically engineered, stem-cell-derived cellular vaccine
    Article Snippet: The cells were removed from culture flasks using trypsin, washed, and transfected with plasmid DNA containing either MICA, MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). .. After 48 h of culture, transfected cells were stained with aqua dye for live/dead discrimination and corresponding antibodies- MICA/MICB (Clone 6D4, PE, BioLegend) or ULBP-1 (clone 170,818, PE, R & D Systems). ..

    Staining:

    Article Title: A genetically engineered, stem-cell-derived cellular vaccine
    Article Snippet: The cells were removed from culture flasks using trypsin, washed, and transfected with plasmid DNA containing either MICA, MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). .. After 48 h of culture, transfected cells were stained with aqua dye for live/dead discrimination and corresponding antibodies- MICA/MICB (Clone 6D4, PE, BioLegend) or ULBP-1 (clone 170,818, PE, R & D Systems). ..



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    Transcriptomic profiling reveals upregulation of <t>ULBP1</t> and ferroptosis-related genes following Erastin treatment. (A) Volcano plots showing differentially expressed genes in KELLY and SK-N-AS neuroblastoma cell lines treated with 1 µM and 2 µM Erastin for 24 hours, respectively, compared to DMSO control. Non-significant genes are shown in gray; significantly upregulated and downregulated genes are shown in red and blue, respectively. (B) KEGG pathway enrichment analysis of DEGs in KELLY and SK-N-AS cells, showing significantly enriched pathways including ferroptosis and amino acid metabolism. (C) Gene Ontology (GO) enrichment network highlighting clusters associated with oxidative stress response, amino acid metabolic processes, and signaling receptor binding, with key genes indicated.
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    Transcriptomic profiling reveals upregulation of <t>ULBP1</t> and ferroptosis-related genes following Erastin treatment. (A) Volcano plots showing differentially expressed genes in KELLY and SK-N-AS neuroblastoma cell lines treated with 1 µM and 2 µM Erastin for 24 hours, respectively, compared to DMSO control. Non-significant genes are shown in gray; significantly upregulated and downregulated genes are shown in red and blue, respectively. (B) KEGG pathway enrichment analysis of DEGs in KELLY and SK-N-AS cells, showing significantly enriched pathways including ferroptosis and amino acid metabolism. (C) Gene Ontology (GO) enrichment network highlighting clusters associated with oxidative stress response, amino acid metabolic processes, and signaling receptor binding, with key genes indicated.
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    Image Search Results


    Transcriptomic profiling reveals upregulation of ULBP1 and ferroptosis-related genes following Erastin treatment. (A) Volcano plots showing differentially expressed genes in KELLY and SK-N-AS neuroblastoma cell lines treated with 1 µM and 2 µM Erastin for 24 hours, respectively, compared to DMSO control. Non-significant genes are shown in gray; significantly upregulated and downregulated genes are shown in red and blue, respectively. (B) KEGG pathway enrichment analysis of DEGs in KELLY and SK-N-AS cells, showing significantly enriched pathways including ferroptosis and amino acid metabolism. (C) Gene Ontology (GO) enrichment network highlighting clusters associated with oxidative stress response, amino acid metabolic processes, and signaling receptor binding, with key genes indicated.

    Journal: Frontiers in Immunology

    Article Title: Erastin-induced ferroptosis enhances natural killer cell anti-tumor activity and offers therapeutic potential in neuroblastoma

    doi: 10.3389/fimmu.2026.1739503

    Figure Lengend Snippet: Transcriptomic profiling reveals upregulation of ULBP1 and ferroptosis-related genes following Erastin treatment. (A) Volcano plots showing differentially expressed genes in KELLY and SK-N-AS neuroblastoma cell lines treated with 1 µM and 2 µM Erastin for 24 hours, respectively, compared to DMSO control. Non-significant genes are shown in gray; significantly upregulated and downregulated genes are shown in red and blue, respectively. (B) KEGG pathway enrichment analysis of DEGs in KELLY and SK-N-AS cells, showing significantly enriched pathways including ferroptosis and amino acid metabolism. (C) Gene Ontology (GO) enrichment network highlighting clusters associated with oxidative stress response, amino acid metabolic processes, and signaling receptor binding, with key genes indicated.

    Article Snippet: Separately, NB cells (KELLY, SK-N-AS, SK-N-BE (2), and SK-N-FI) were seeded and washed with PBS, then stained for 30 minutes at 4°C with anti-ULBP1 primary antibody (1:100, clone 170818, R&D systems, #MAB-1380-500) prior to washing and 30 min incubation with secondary antibody (1:500, Invitrogen, # A55747 ).

    Techniques: Control, Binding Assay

    Low ULBP1 expression correlates with poor prognosis in neuroblastoma patients from the SEQC cohort. Kaplan–Meier overall survival analysis was performed using the SEQC neuroblastoma dataset (n = 498) via the R2 Genomics Analysis and Visualization Platform (A) Complete cohort; (B) MYCN -amplified subset; (C) Patients with progressive disease; (D) INSS stage 4 subset.

    Journal: Frontiers in Immunology

    Article Title: Erastin-induced ferroptosis enhances natural killer cell anti-tumor activity and offers therapeutic potential in neuroblastoma

    doi: 10.3389/fimmu.2026.1739503

    Figure Lengend Snippet: Low ULBP1 expression correlates with poor prognosis in neuroblastoma patients from the SEQC cohort. Kaplan–Meier overall survival analysis was performed using the SEQC neuroblastoma dataset (n = 498) via the R2 Genomics Analysis and Visualization Platform (A) Complete cohort; (B) MYCN -amplified subset; (C) Patients with progressive disease; (D) INSS stage 4 subset.

    Article Snippet: Separately, NB cells (KELLY, SK-N-AS, SK-N-BE (2), and SK-N-FI) were seeded and washed with PBS, then stained for 30 minutes at 4°C with anti-ULBP1 primary antibody (1:100, clone 170818, R&D systems, #MAB-1380-500) prior to washing and 30 min incubation with secondary antibody (1:500, Invitrogen, # A55747 ).

    Techniques: Expressing, Amplification

    Erastin treatment increases ULBP1 surface expression and enhances NK cell-mediated cytotoxicity in neuroblastoma cells. (A) Surface expression of ULBP1 was measured by flow cytometry in KELLY, SK-N-AS, SK-N-BE (2), and SK-N-FI cells treated with DMSO (control) or Erastin. (B) NK cell-mediated cytotoxicity assay using NK-92 cells co-cultured with neuroblastoma cells (E:T ratio was 8:1) pre-treated with either DMSO (Control), Erastin, or Erastin in combination with Liproxstatin-1 (Lip-1). Cell lysis percentages were quantified in KELLY, SK-N-AS, and SK-N-FI cells. n = 3 biologically independent experiments. *P<0.05, **P<0.01, ***P<0.001; Paired t test.

    Journal: Frontiers in Immunology

    Article Title: Erastin-induced ferroptosis enhances natural killer cell anti-tumor activity and offers therapeutic potential in neuroblastoma

    doi: 10.3389/fimmu.2026.1739503

    Figure Lengend Snippet: Erastin treatment increases ULBP1 surface expression and enhances NK cell-mediated cytotoxicity in neuroblastoma cells. (A) Surface expression of ULBP1 was measured by flow cytometry in KELLY, SK-N-AS, SK-N-BE (2), and SK-N-FI cells treated with DMSO (control) or Erastin. (B) NK cell-mediated cytotoxicity assay using NK-92 cells co-cultured with neuroblastoma cells (E:T ratio was 8:1) pre-treated with either DMSO (Control), Erastin, or Erastin in combination with Liproxstatin-1 (Lip-1). Cell lysis percentages were quantified in KELLY, SK-N-AS, and SK-N-FI cells. n = 3 biologically independent experiments. *P<0.05, **P<0.01, ***P<0.001; Paired t test.

    Article Snippet: Separately, NB cells (KELLY, SK-N-AS, SK-N-BE (2), and SK-N-FI) were seeded and washed with PBS, then stained for 30 minutes at 4°C with anti-ULBP1 primary antibody (1:100, clone 170818, R&D systems, #MAB-1380-500) prior to washing and 30 min incubation with secondary antibody (1:500, Invitrogen, # A55747 ).

    Techniques: Expressing, Flow Cytometry, Control, Cytotoxicity Assay, Cell Culture, Lysis